Renewable Feedstock
Our Technology
Enzymit is transitioning
bioproduction to Cell-Free Production
PRESENT
Fermentation based bioproduction
Fermentation-based bioproduction relies on living cells, making it complex, costly, and time-consuming.
FUTURE
Cell-free manufacturing
Cell-free manufacturing offers a simpler, faster, and more efficient process by using enzymes to convert basic compounds into specialized chemicals.
Discover the Future
PRESENT
Fermentation based bioproduction
Fermentation-based bioproduction relies on living cells, making it complex, costly, and time-consuming.
FUTURE
Cell-free manufacturing
Cell-free manufacturing offers a simpler, faster, and more efficient process by using enzymes to convert basic compounds into specialized chemicals.
PRESENT
Fermentation based bioproduction
Fermentation-based bioproduction relies on living cells, making it complex, costly, and time-consuming.
FUTURE
Cell-free manufacturing
Cell-free manufacturing offers a simpler, faster, and more efficient process by using enzymes to convert basic compounds into specialized chemicals.
PRESENT
Fermentation based bioproduction
Fermentation-based bioproduction relies on living cells, making it complex, costly, and time-consuming.
FUTURE
Cell-free manufacturing
Cell-free manufacturing offers a simpler, faster, and more efficient process by using enzymes to convert basic compounds into specialized chemicals.
Enzymit is
transitioning
bioproduction to Cell-Free Production
Cell-free. The New
Bio-Production Standard
Cell-free. The New
Bio-Production Standard
Cell-Free Bioreactor is more sustainable and efficient than traditional fermentation and chemical manufacturing.
Cell-free.
The New
Bio-Production Standard
Cell-Free Bioreactor is more sustainable and efficient than traditional fermentation and chemical manufacturing.
Cell-Free Bioreactor
Cell-Free Bioreactor
Cell-Free Bioreactor
Sustainability
Efficiency
Sustainability
Efficiency
Chemical Manufacturing
Chemical Manufacturing
Fermentation
Fermentation
Renewable Feedstock
Enzymatic Processing
Enzymatic Processing
Purification & Formulation
Purification & Formulation
Output product
Output product
Renewable Feedstock
Enzymatic Processing
Purification & Formulation
Output product
Renewable Feedstock
Enzymatic Processing
Purification & Formulation
Output product
ENZYMIT Enables the Cell-Free Manufacturing Revolution with Cutting-Edge Enzyme Design Technology
ENZYMIT Enables the Cell-Free
Manufacturing Revolution with Cutting-Edge Enzyme Design Technology
ENZYMIT Enables the Cell-Free
Manufacturing Revolution with Cutting-Edge Enzyme Design Technology
SolvIT - High protein expression in E.coli
E.coli soluble sexpression
SolvIT V1
SolvIT V2
Human Derived Fucosyl transferase
Soluble titer
V1
V2
V3
V4
V5
Expression level (g/L)
15
10
5
0
Protein expression in bacterial host is low-2-no for most proteins
ENZYMIT’s SolvIT GNN works as an optimization layer on top of our design enegins
ThermIT - Extreme Protein stabilization
Mutations are chosen by the ThermIT
Iron sulfur cluster and citrate substrate
Tyrosine improves packing
Proline rigidifies loop
Proline rigidifies loop
Continuous improvement of algorithm performance
Wild Type
v1
v2
v3
v4
Our AI Powered algorithm can take any protein sequence, introduce dozens of stabilizing mutations in single shot design.
We have tested the algorithm on multiple proteins and enzyme types.
AFGQWVNMDRLC
Input sequence
Attn. map
AFGQWVNMDRLWC
AFQPCLLNMDRLC
AFGQWVCARFQYC
AVGLMMVNMDRLC
Output sequence
CoSaNN - Conformation Sampling using Neural Networks
Inorganic phosphate
¢
Design
ATP
$$$
WT
We can design the conformations of enzymes so they can accept non-natural molecule. This is crucial for replacing expensive cofactors
Novel inorganic phosphate dependant kinase
CoSaNN - Conformation Sampling using Neural Networks
Within our designed bioreactor enzyme are immobilized to a support resin
Immobilizing enzymes to a support resin increases the enzymes inherit stability and enables multiple use cycles, both are crucial to reach economic viability
SolvIT - High protein expression in E.coli
E.coli soluble sexpression
SolvIT V1
SolvIT V2
Human Derived Fucosyl transferase
Soluble titer
Jan
22
Feb
22
Mar
22
May
22
Jul
22
Expression level (g/L)
15
10
5
0
Protein expression in bacterial host is low-2-no for most proteins.
ENZYMIT’s SolvIT GNN works as an optimization layer on top of our design enegins.
SolvIT - High protein expression in E.coli
E.coli soluble sexpression
SolvIT V1
SolvIT V2
Human Derived Fucosyl transferase
Soluble titer
Jan
22
Feb
22
Mar
22
May
22
Jul
22
Expression level (g/L)
15
10
5
0
Protein expression in bacterial host is low-2-no for most proteins.
ENZYMIT’s SolvIT GNN works as an optimization layer on top of our design enegins.
SolvIT - High protein expression in E.coli
E.coli soluble sexpression
SolvIT V1
SolvIT V2
Human Derived Fucosyl transferase
Soluble titer
V1
V2
V3
V4
V5
Expression level (g/L)
15
10
5
0
Protein expression in bacterial host is low-2-no for most proteins.
ENZYMIT’s SolvIT GNN works as an optimization layer on top of our design enegins.
Key advantages of
Enzymit’s cell-free bioproduction
Key advantages of
Enzymit’s cell-free
bioproduction
Key advantages of Enzymit’s cell-free
bioproduction
Simple and Flexible
Removes complexity of working with live cells. Scale-up simpler and more flexible.
Higher Yields
Cell-free production yields greatly exceed fermentation.
Unique Compounds
Cell-free process can create fermentation-unattainable
compounds, such as
antibiotics and size-limiting molecules.
Reduced Cost
Continuous bioreactors provide significantly more output with a vastly smaller footprint.
Simple and Flexible
Removes complexity of working with live cells. Scale-up simpler and more flexible.
Higher Yields
Cell-free production yields greatly exceed fermentation.
Unique Compounds
Cell-free process can create fermentation-unattainable
compounds, such as
antibiotics and size-limiting molecules.
Reduced Cost
Continuous bioreactors provide significantly more output with a vastly smaller footprint.
Simple and Flexible
Higher Yields
Unique Compounds
Reduced Cost
Bio Manufacturing
Bio
Manufacturing
Made faster, cheaper and scalable
Powered by artificial intelligence, Enzymit designs and creates novel enzymes to design cell-free production in three ways
Process Design
Includes enzyme immobilization, bioreactor design and down stream-processing.
Enzyme Production
Fermentation process development for efficient low-cost enzyme production.
A
B
C
D
A
D
Cascade Design
Simplified biochemical pathways to enable streamlined industrial processes and avoid costly cofactors and coenzymes.
Enzyme Design
New-to-nature enzymes catalyze novel functions are highly-stable and cheap to produce.
Process Design
Includes enzyme immobilization, bioreactor design and down stream-processing.
Enzyme Production
Fermentation process development for efficient low-cost enzyme production.
A
B
C
D
A
D
Cascade Design
Simplified biochemical pathways to enable streamlined industrial processes and avoid costly cofactors and coenzymes.
Enzyme Design
New-to-nature enzymes catalyze novel functions are highly-stable and cheap to produce.
Process Design
Includes enzyme immobilization, bioreactor design and down stream-processing.
Enzyme Production
Fermentation process development for efficient low-cost enzyme production.
A
B
C
D
A
D
Cascade Design
Simplified biochemical pathways to enable streamlined industrial processes and avoid costly cofactors and coenzymes.
Enzyme Design
New-to-nature enzymes catalyze novel functions are highly-stable and cheap to produce.
Novel Enzyme Design
Enzymit’s proprietary enzyme design platform utilizes advanced computational and deep learning algorithms, combined with sophisticated experimental tools, to create highly efficient and cost-effective solutions for cell-free enzymatic manufacturing.
Our technology allows us to design enzymes with high titer expressions and exceptionally stable properties that can withstand higher temperatures and be reused in production cycles. The platform leverages more cost-effective molecules to drive chemical synthesis, making our approach both sustainable and economically feasible.
Enzymit's
Technology Platform
Enzymit's
Technology Platform
This comprehensive approach enables us to develop ever more efficient and cost-effective solutions for cell-free enzymatic manufacturing.
This comprehensive approach enables us to develop ever more efficient and cost- effective solutions for cell-free enzymatic manufacturing.
This comprehensive approach enables us to develop ever more efficient and cost-effective solutions for cell-free enzymatic manufacturing.
Design
Proprietary algorithms and deep neural network models enable the computational design of enzymes, generating millions of candidates.
Build
Application of an additional suite of algorithms to adapt enzymes for high-throughput DNA assembly, enabling the creation of billions of DNA sequences coded specifically for the designed enzymes.
Test
Experimental, high-throughput screening and advanced analytical methods can test billions of sequence changes in a single experiment to evaluate and identify the best candidates.
Learn
The output of each round serves as an additional data set used to train the next generation of algorithms, continuously improving and optimizing our technology platform for even better enzyme design and production.
Design
Proprietary algorithms and deep neural network models enable the computational design of enzymes, generating millions of candidates.
Build
Application of an additional suite of algorithms to adapt enzymes for high-throughput DNA assembly, enabling the creation of billions of DNA sequences coded specifically for the designed enzymes.
Test
Experimental, high-throughput screening and advanced analytical methods can test billions of sequence changes in a single experiment to evaluate and identify the best candidates.
Learn
The output of each round serves as an additional data set used to train the next generation of algorithms, continuously improving and optimizing our technology platform for even better enzyme design and production.
Design
Proprietary algorithms and deep neural network models enable the computational design of enzymes, generating millions of candidates.
Build
Application of an additional suite of algorithms to adapt enzymes for high-throughput DNA assembly, enabling the creation of billions of DNA sequences coded specifically for the designed enzymes.
Test
Experimental, high-throughput screening and advanced analytical methods can test billions of sequence changes in a single experiment to evaluate and identify the best candidates.
Learn
The output of each round serves as an additional data set used to train the next generation of algorithms, continuously improving and optimizing our technology platform for even better enzyme design and production.
Design
Proprietary algorithms and deep neural network models enable the computational design of enzymes, generating millions of candidates.
Build
Application of an additional suite of algorithms to adapt enzymes for high-throughput DNA assembly, enabling the creation of billions of DNA sequences coded specifically for the designed enzymes.
Test
Experimental, high-throughput screening and advanced analytical methods can test billions of sequence changes in a single experiment to evaluate and identify the best candidates.
Learn
The output of each round serves as an additional data set used to train the next generation of algorithms, continuously improving and optimizing our technology platform for even better enzyme design and production.
Design
Proprietary algorithms and deep neural network models enable the computational design of enzymes, generating millions of candidates.
Design
Proprietary algorithms and deep neural network models enable the computational design of enzymes, generating millions of candidates.
Build
Application of an additional suite of algorithms to adapt enzymes for high-throughput DNA assembly, enabling the creation of billions of DNA sequences coded specifically for the designed enzymes.
Build
Application of an additional suite of algorithms to adapt enzymes for high-throughput DNA assembly, enabling the creation of billions of DNA sequences coded specifically for the designed enzymes.
Test
Experimental, high-throughput screening and advanced analytical methods can test billions of sequence changes in a single experiment to evaluate and identify the best candidates.
Test
Experimental, high-throughput screening and advanced analytical methods can test billions of sequence changes in a single experiment to evaluate and identify the best candidates.
Learn
The output of each round serves as an additional data set used to train the next generation of algorithms, continuously improving and optimizing our technology platform for even better enzyme design and production.
Learn
The output of each round serves as an additional data set used to train the next generation of algorithms, continuously improving and optimizing our technology platform for even better enzyme design and production.